| Year | Date | Historical Events |
| 1919 | Apr 11 | Keel laid down by the Bethlehem Shipbuilding Corp.,Quincy, MA. |
| 1922 | Oct 18 | Launched. |
| 1924 | Jan 22 | Commissioned with LT. Theodore Waldschmidt in command.
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| 1925 | Jun 1 | S-27 arrived at her homeport of San Diego,CA. |
| 1931 | Feb 23 | S-27 arrived at Pearl Harbor,HI. |
| 1939 | Jun 16 | S-27 sailed East. |
| | Jun 27 | S-27 arrived at San Diego, CA. |
| 1941 | Nov 30 | S-27 proceeded to the Mare Island Naval Shipyard, Vallejo, CA., where she was undergoing overhaul when the United States entered World War II.
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| 1942 | Jan 23 | S-27 departed San Francisco,CA. |
| | Jan 26 | S-27 arrived at San Diego,CA. She resumed operations for the Sound School which she continued into the spring. |
| | May 20 | S-27 departed San Diego, CA. for the Aleutian Islands. |
| | Jun 12 | A little over a week after the beginning of the war in the Aleutians, S-27 put into Dutch Harbor, took on provisions, refueled, and then headed West with orders to patrol in the Kuluk Bay area and to reconnoiter Constantine Harbor, Amchitka. |
| | Jun 18 | S-27 reconnoitered Constantine Harbor found no signs of enemy activity in that evacuated village, and moved on to round the southern end of the island, whence she would proceed to Kiska. |
| | Jun 19 | Soon after 0043 on the 19th, breakers were sighted about 25 yards forward of the bow. "Back emergency" orders were given. Seconds later, S-27 grounded on rocks off St. Makarius Point.
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| | Jun 24 | S-27 was abandonded and the crew was rescued by PBY aircraft. |